mouse reg3b antibody Search Results


anti reg3β  (R&D Systems)
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R&D Systems anti reg3β
Anti Reg3β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti reg3β/product/R&D Systems
Average 94 stars, based on 1 article reviews
anti reg3β - by Bioz Stars, 2026-03
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rat anti mouse reg3b antibodies  (R&D Systems)
93
R&D Systems rat anti mouse reg3b antibodies
Western blot analysis of <t>Reg3b</t> and Reg3g in pooled ileal mucosa samples of Reg3b−/− mice (KO) or WT mice (Wt). Reg3b−/− mice (n = 10) and WT mice (n = 12) were orally infected with S. enteritidis (S), or Reg3b−/− mice (n = 7) and WT mice (n = 8) were orally infected with L. monocytogenes (L). Noninfected mice (n = 5) received saline as a control treatment.
Rat Anti Mouse Reg3b Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti mouse reg3b antibodies/product/R&D Systems
Average 93 stars, based on 1 article reviews
rat anti mouse reg3b antibodies - by Bioz Stars, 2026-03
93/100 stars
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anti green fluorescent protein gfp  (R&D Systems)
93
R&D Systems anti green fluorescent protein gfp
Expression of Reg3β and infiltration of RORγt + cells in the small intestine of CFLARs Tg mice at the embryonic stage. (A, B) Small intestine sections from wild-type (WT), X CF Y , and X CF X mice on a <t>Rorc-gfp</t> /+ genetic background at E18.5 were stained with <t>anti-GFP</t> (green) and anti-Reg3β (red) (A), or anti-pSTAT3 antibodies (magenta) (B). Lower panels are enlarged images of white boxes in the upper panels (A). White arrowheads indicate RORγt + cells. Scale bars, 100 μm. (C) Expression of Reg3β in the small intestine and colon of adult wild-type mice. Tissue extracts were prepared from the small intestine and colon of 8- to 12-week-old wild-type mice and examined by Western blotting with the indicated antibodies. Each number indicates an individual mouse. Results represent two independent experiments. (D) mRNA was prepared from the small intestine and colon of 8- to 12-week-old mice, and the expression of Reg3b and Reg3g was determined by qPCR. Results are mean ± SEM (n = 12 mice). Statistical significance was determined using the two-tailed unpaired Student's t -test. *** P < 0.001.
Anti Green Fluorescent Protein Gfp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti green fluorescent protein gfp/product/R&D Systems
Average 93 stars, based on 1 article reviews
anti green fluorescent protein gfp - by Bioz Stars, 2026-03
93/100 stars
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anti reg3b  (R&D Systems)
92
R&D Systems anti reg3b
Effect of Reg3g deficiency on bacterial translocation. (A) Results of anaerobic mesenteric lymph node cultures and total bacteria in liver specimens. (B) Plasma lipopolysaccharide levels. (C) Expression of Tlr4 in liver samples. (D) <t>REG3B</t> protein expression in ileal samples. (E) Representative REG3B expression in ileal samples of FFD‐fed WT and Reg3g −/− mice. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates. Abbreviation: KO, knockout.
Anti Reg3b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti reg3b/product/R&D Systems
Average 92 stars, based on 1 article reviews
anti reg3b - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier

Image Search Results


Western blot analysis of Reg3b and Reg3g in pooled ileal mucosa samples of Reg3b−/− mice (KO) or WT mice (Wt). Reg3b−/− mice (n = 10) and WT mice (n = 12) were orally infected with S. enteritidis (S), or Reg3b−/− mice (n = 7) and WT mice (n = 8) were orally infected with L. monocytogenes (L). Noninfected mice (n = 5) received saline as a control treatment.

Journal: Infection and Immunity

Article Title: Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

doi: 10.1128/IAI.06165-11

Figure Lengend Snippet: Western blot analysis of Reg3b and Reg3g in pooled ileal mucosa samples of Reg3b−/− mice (KO) or WT mice (Wt). Reg3b−/− mice (n = 10) and WT mice (n = 12) were orally infected with S. enteritidis (S), or Reg3b−/− mice (n = 7) and WT mice (n = 8) were orally infected with L. monocytogenes (L). Noninfected mice (n = 5) received saline as a control treatment.

Article Snippet: Proteins were transferred to an Immobilon-FL membrane (Millipore) and, after blocking, exposed to rat anti-mouse Reg3b antibodies (MAB5110, 1:2,000; R&D Systems, Minneapolis).

Techniques: Western Blot, Infection, Saline, Control

Salmonella (triangles) and Listeria (circles) excretion in feces. Individually housed mice were orally infected at day 0. Reg3b−/− mice (filled symbols) were infected with 5 × 108 CFU of S. enteritidis (n = 10) or L. monocytogenes (n = 7), and WT littermates (open symbols) were infected with identical numbers of CFU of S. enteritidis (n = 12) or L. monocytogenes (n = 8). Values are the means and SEM.

Journal: Infection and Immunity

Article Title: Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

doi: 10.1128/IAI.06165-11

Figure Lengend Snippet: Salmonella (triangles) and Listeria (circles) excretion in feces. Individually housed mice were orally infected at day 0. Reg3b−/− mice (filled symbols) were infected with 5 × 108 CFU of S. enteritidis (n = 10) or L. monocytogenes (n = 7), and WT littermates (open symbols) were infected with identical numbers of CFU of S. enteritidis (n = 12) or L. monocytogenes (n = 8). Values are the means and SEM.

Article Snippet: Proteins were transferred to an Immobilon-FL membrane (Millipore) and, after blocking, exposed to rat anti-mouse Reg3b antibodies (MAB5110, 1:2,000; R&D Systems, Minneapolis).

Techniques: Infection

Levels of Salmonella (A and B) and Listeria (C and D) in intestinal and extraintestinal tissues at 4 and 2 days after oral infection, respectively. Numbers of CFU of pathogens in the ileal and colonic mucosas (A and C) and in mesenteric lymph nodes (MLN), spleen, and liver (B and D) of Reg3b−/− mice (n = 10; black bars) or WT mice (n = 12; white bars). Values are the means and SEM. An asterisk indicates that the results are significantly different from those of the WT (P < 0.05).

Journal: Infection and Immunity

Article Title: Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

doi: 10.1128/IAI.06165-11

Figure Lengend Snippet: Levels of Salmonella (A and B) and Listeria (C and D) in intestinal and extraintestinal tissues at 4 and 2 days after oral infection, respectively. Numbers of CFU of pathogens in the ileal and colonic mucosas (A and C) and in mesenteric lymph nodes (MLN), spleen, and liver (B and D) of Reg3b−/− mice (n = 10; black bars) or WT mice (n = 12; white bars). Values are the means and SEM. An asterisk indicates that the results are significantly different from those of the WT (P < 0.05).

Article Snippet: Proteins were transferred to an Immobilon-FL membrane (Millipore) and, after blocking, exposed to rat anti-mouse Reg3b antibodies (MAB5110, 1:2,000; R&D Systems, Minneapolis).

Techniques: Infection

Listeria and Salmonella pulldown assays. Presence of Reg3b, detected by Western blot analysis, in supernatant fractions of ileal mucosa samples from S. enteritidis-infected WT (Wt) or Reg3b−/− (KO) mice incubated with Salmonella (S) or Listeria (L). Mucosal samples incubated without bacteria served as the negative control (−). The prestained protein marker is indicated by M.

Journal: Infection and Immunity

Article Title: Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

doi: 10.1128/IAI.06165-11

Figure Lengend Snippet: Listeria and Salmonella pulldown assays. Presence of Reg3b, detected by Western blot analysis, in supernatant fractions of ileal mucosa samples from S. enteritidis-infected WT (Wt) or Reg3b−/− (KO) mice incubated with Salmonella (S) or Listeria (L). Mucosal samples incubated without bacteria served as the negative control (−). The prestained protein marker is indicated by M.

Article Snippet: Proteins were transferred to an Immobilon-FL membrane (Millipore) and, after blocking, exposed to rat anti-mouse Reg3b antibodies (MAB5110, 1:2,000; R&D Systems, Minneapolis).

Techniques: Western Blot, Infection, Incubation, Bacteria, Negative Control, Marker

Expression of Reg3β and infiltration of RORγt + cells in the small intestine of CFLARs Tg mice at the embryonic stage. (A, B) Small intestine sections from wild-type (WT), X CF Y , and X CF X mice on a Rorc-gfp /+ genetic background at E18.5 were stained with anti-GFP (green) and anti-Reg3β (red) (A), or anti-pSTAT3 antibodies (magenta) (B). Lower panels are enlarged images of white boxes in the upper panels (A). White arrowheads indicate RORγt + cells. Scale bars, 100 μm. (C) Expression of Reg3β in the small intestine and colon of adult wild-type mice. Tissue extracts were prepared from the small intestine and colon of 8- to 12-week-old wild-type mice and examined by Western blotting with the indicated antibodies. Each number indicates an individual mouse. Results represent two independent experiments. (D) mRNA was prepared from the small intestine and colon of 8- to 12-week-old mice, and the expression of Reg3b and Reg3g was determined by qPCR. Results are mean ± SEM (n = 12 mice). Statistical significance was determined using the two-tailed unpaired Student's t -test. *** P < 0.001.

Journal: Biochemistry and Biophysics Reports

Article Title: Regenerating islet-derived protein (Reg)3β plays a crucial role in attenuation of ileitis and colitis in mice

doi: 10.1016/j.bbrep.2020.100738

Figure Lengend Snippet: Expression of Reg3β and infiltration of RORγt + cells in the small intestine of CFLARs Tg mice at the embryonic stage. (A, B) Small intestine sections from wild-type (WT), X CF Y , and X CF X mice on a Rorc-gfp /+ genetic background at E18.5 were stained with anti-GFP (green) and anti-Reg3β (red) (A), or anti-pSTAT3 antibodies (magenta) (B). Lower panels are enlarged images of white boxes in the upper panels (A). White arrowheads indicate RORγt + cells. Scale bars, 100 μm. (C) Expression of Reg3β in the small intestine and colon of adult wild-type mice. Tissue extracts were prepared from the small intestine and colon of 8- to 12-week-old wild-type mice and examined by Western blotting with the indicated antibodies. Each number indicates an individual mouse. Results represent two independent experiments. (D) mRNA was prepared from the small intestine and colon of 8- to 12-week-old mice, and the expression of Reg3b and Reg3g was determined by qPCR. Results are mean ± SEM (n = 12 mice). Statistical significance was determined using the two-tailed unpaired Student's t -test. *** P < 0.001.

Article Snippet: The following antibodies used in this study were obtained from the indicated sources: anti-green fluorescent protein (GFP) (Go-Af1480, Frontier Institute), anti-Reg3β (AF5110, R&D Systems), anti-Reg3γ (provided by H. Kiyama), anti-phospho-STAT3 (9131, Cell Signaling), anti-STAT3 (sc-482, Santa Cruz), anti-β-tubulin (T5168, Sigma-Aldrich), anti-CD45.2 (104, BioLegend), anti-CD11b (M1/70, TONBO Biosciences), and anti-Ly-6G (1A8, TONBO Biosciences).

Techniques: Expressing, Staining, Western Blot, Two Tailed Test

Effect of Reg3g deficiency on bacterial translocation. (A) Results of anaerobic mesenteric lymph node cultures and total bacteria in liver specimens. (B) Plasma lipopolysaccharide levels. (C) Expression of Tlr4 in liver samples. (D) REG3B protein expression in ileal samples. (E) Representative REG3B expression in ileal samples of FFD‐fed WT and Reg3g −/− mice. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates. Abbreviation: KO, knockout.

Journal: Hepatology Communications

Article Title: The Role of Intestinal C‐type Regenerating Islet Derived‐3 Lectins for Nonalcoholic Steatohepatitis

doi: 10.1002/hep4.1165

Figure Lengend Snippet: Effect of Reg3g deficiency on bacterial translocation. (A) Results of anaerobic mesenteric lymph node cultures and total bacteria in liver specimens. (B) Plasma lipopolysaccharide levels. (C) Expression of Tlr4 in liver samples. (D) REG3B protein expression in ileal samples. (E) Representative REG3B expression in ileal samples of FFD‐fed WT and Reg3g −/− mice. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates. Abbreviation: KO, knockout.

Article Snippet: We used 50 μg of protein homogenate for immunoblotting and the following primary antibodies: anti‐Reg3b (MAB5110; R&D Systems, Inc., Minneapolis, MN), anti‐Reg3g, and anti‐β‐actin (A5441; Sigma‐Aldrich).

Techniques: Translocation Assay, Bacteria, Expressing, Knock-Out

Effect of Reg3b deficiency on body weight and metabolic function. (A) Body weight and relative weights of epididymal and brown fat tissues after 20 weeks of feeding. (B) Food intake per group over the course of the experiment (C) Glucose tolerance test performed after 18 weeks of feeding. (D) Insulin tolerance test performed after 19 weeks of feeding. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates. Abbreviations: AOB, area over baseline; AUC, area under the curve.

Journal: Hepatology Communications

Article Title: The Role of Intestinal C‐type Regenerating Islet Derived‐3 Lectins for Nonalcoholic Steatohepatitis

doi: 10.1002/hep4.1165

Figure Lengend Snippet: Effect of Reg3b deficiency on body weight and metabolic function. (A) Body weight and relative weights of epididymal and brown fat tissues after 20 weeks of feeding. (B) Food intake per group over the course of the experiment (C) Glucose tolerance test performed after 18 weeks of feeding. (D) Insulin tolerance test performed after 19 weeks of feeding. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates. Abbreviations: AOB, area over baseline; AUC, area under the curve.

Article Snippet: We used 50 μg of protein homogenate for immunoblotting and the following primary antibodies: anti‐Reg3b (MAB5110; R&D Systems, Inc., Minneapolis, MN), anti‐Reg3g, and anti‐β‐actin (A5441; Sigma‐Aldrich).

Techniques:

Effect of Reg3b deficiency on liver phenotype. (A) AST plasma levels. (B) Representative hematoxylin and eosin‐stained liver sections. (C‐E) Expression of inflammatory genes in liver samples. (F) Total amount of triglycerides per liver. (G) Relative liver weight. (H) Total amount of hydroxyproline per liver. (I) Representative sirius red‐stained liver sections. (J,K) Expression of fibrosis‐related genes in liver samples. Scale bars, 50 μm. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates.

Journal: Hepatology Communications

Article Title: The Role of Intestinal C‐type Regenerating Islet Derived‐3 Lectins for Nonalcoholic Steatohepatitis

doi: 10.1002/hep4.1165

Figure Lengend Snippet: Effect of Reg3b deficiency on liver phenotype. (A) AST plasma levels. (B) Representative hematoxylin and eosin‐stained liver sections. (C‐E) Expression of inflammatory genes in liver samples. (F) Total amount of triglycerides per liver. (G) Relative liver weight. (H) Total amount of hydroxyproline per liver. (I) Representative sirius red‐stained liver sections. (J,K) Expression of fibrosis‐related genes in liver samples. Scale bars, 50 μm. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates.

Article Snippet: We used 50 μg of protein homogenate for immunoblotting and the following primary antibodies: anti‐Reg3b (MAB5110; R&D Systems, Inc., Minneapolis, MN), anti‐Reg3g, and anti‐β‐actin (A5441; Sigma‐Aldrich).

Techniques: Staining, Expressing

Effect of Reg3b deficiency on bacterial translocation. (A) Total bacteria in liver specimens. (B) Plasma LPS levels. (C) Expression of Tlr4 in liver samples. (D) REG3G protein expression in ileal samples. (E) Representative REG3G expression in ileal samples of FFD‐fed WT and Reg3g −/− mice. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates. Abbreviation: KO, knockout.

Journal: Hepatology Communications

Article Title: The Role of Intestinal C‐type Regenerating Islet Derived‐3 Lectins for Nonalcoholic Steatohepatitis

doi: 10.1002/hep4.1165

Figure Lengend Snippet: Effect of Reg3b deficiency on bacterial translocation. (A) Total bacteria in liver specimens. (B) Plasma LPS levels. (C) Expression of Tlr4 in liver samples. (D) REG3G protein expression in ileal samples. (E) Representative REG3G expression in ileal samples of FFD‐fed WT and Reg3g −/− mice. Data represent mean ± SEM; * P < 0.05. Refer to http://onlinelibrary.wiley.com/doi/10.1002/hep4.1165/full for numbers of biological replicates. Abbreviation: KO, knockout.

Article Snippet: We used 50 μg of protein homogenate for immunoblotting and the following primary antibodies: anti‐Reg3b (MAB5110; R&D Systems, Inc., Minneapolis, MN), anti‐Reg3g, and anti‐β‐actin (A5441; Sigma‐Aldrich).

Techniques: Translocation Assay, Bacteria, Expressing, Knock-Out